Ontology highlight
ABSTRACT:
SUBMITTER: Wozniak-Knopp G
PROVIDER: S-EPMC5891013 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Wozniak-Knopp Gordana G Stadlmayr Gerhard G Perthold Jan Walther JW Stadlbauer Katharina K Gotsmy Mathias M Becker Stefan S Rüker Florian F
PloS one 20180409 4
We have designed a complete antibody-like construct where the CH1 and Cκ domains are exchanged for a pair of the CH3 domains and efficient pairing of the heavy and light variable domain is achieved using "Knobs-into-Holes" strategy. This construct, composed of only naturally occurring immunoglobulin sequences without artificial linkers, expressed at a high level in mammalian cells, however exhibited low solubility. Rational mutagenesis aimed at the amino acid residues located at the interface of ...[more]