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A synthetic pathway for the fixation of carbon dioxide in vitro.

ABSTRACT: Carbon dioxide (CO2) is an important carbon feedstock for a future green economy. This requires the development of efficient strategies for its conversion into multicarbon compounds. We describe a synthetic cycle for the continuous fixation of CO2 in vitro. The crotonyl-coenzyme A (CoA)/ethylmalonyl-CoA/hydroxybutyryl-CoA (CETCH) cycle is a reaction network of 17 enzymes that converts CO2 into organic molecules at a rate of 5 nanomoles of CO2 per minute per milligram of protein. The CETCH cycle was drafted by metabolic retrosynthesis, established with enzymes originating from nine different organisms of all three domains of life, and optimized in several rounds by enzyme engineering and metabolic proofreading. The CETCH cycle adds a seventh, synthetic alternative to the six naturally evolved CO2 fixation pathways, thereby opening the way for in vitro and in vivo applications.

SUBMITTER: Schwander T

PROVIDER: S-EPMC5892708 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature

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A synthetic pathway for the fixation of carbon dioxide in vitro.

Schwander Thomas T Schada von Borzyskowski Lennart L Burgener Simon S Cortina Niña Socorro NS Erb Tobias J TJ

Science (New York, N.Y.) 20161101 6314

Carbon dioxide (CO2) is an important carbon feedstock for a future green economy. This requires the development of efficient strategies for its conversion into multicarbon compounds. We describe a synthetic cycle for the continuous fixation of CO2 in vitro. The crotonyl-coenzyme A (CoA)/ethylmalonyl-CoA/hydroxybutyryl-CoA (CETCH) cycle is a reaction network of 17 enzymes that converts CO2 into organic molecules at a rate of 5 nanomoles of CO2 per minut ...[more]

PMID: 27856910

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