Ontology highlight
ABSTRACT:
SUBMITTER: Payne JT
PROVIDER: S-EPMC5898188 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Journal of the American Chemical Society 20180108 2
Extensive effort has been devoted to engineering flavin-dependent halogenases (FDHs) with improved stability, expanded substrate scope, and altered regioselectivity. Here, we show that variants of rebeccamycin halogenase (RebH) catalyze enantioselective desymmetrization of methylenedianilines via halogenation of these substrates distal to their pro-stereogenic center. Structure-guided engineering was used to increase the conversion and selectivity of these reactions, and the synthetic utility of ...[more]