Unknown

Dataset Information

0

Aromatic Halogenation by Using Bifunctional Flavin Reductase-Halogenase Fusion Enzymes.


ABSTRACT: The remarkable site selectivity and broad substrate scope of flavin-dependent halogenases (FDHs) has led to much interest in their potential as biocatalysts. Multiple engineering efforts have demonstrated that FDHs can be tuned for non-native substrate scope and site selectivity. FDHs have also proven useful as in vivo biocatalysts and have been successfully incorporated into biosynthetic pathways to build new chlorinated aromatic compounds in several heterologous organisms. In both cases, reduced flavin cofactor, usually supplied by a separate flavin reductase (FR), is required. Herein, we report functional synthetic, fused FDH-FR proteins containing various FDHs and FRs joined by different linkers. We show that FDH-FR fusion proteins can increase product titers compared to the individual components for in vivo biocatalysis in Escherichia coli.

SUBMITTER: Andorfer MC 

PROVIDER: S-EPMC5898195 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

Aromatic Halogenation by Using Bifunctional Flavin Reductase-Halogenase Fusion Enzymes.

Andorfer Mary C MC   Belsare Ketaki D KD   Girlich Anna M AM   Lewis Jared C JC  

Chembiochem : a European journal of chemical biology 20170922 21


The remarkable site selectivity and broad substrate scope of flavin-dependent halogenases (FDHs) has led to much interest in their potential as biocatalysts. Multiple engineering efforts have demonstrated that FDHs can be tuned for non-native substrate scope and site selectivity. FDHs have also proven useful as in vivo biocatalysts and have been successfully incorporated into biosynthetic pathways to build new chlorinated aromatic compounds in several heterologous organisms. In both cases, reduc  ...[more]

Similar Datasets

| S-EPMC9775415 | biostudies-literature
| S-EPMC5659210 | biostudies-literature
| S-EPMC3824154 | biostudies-literature
| S-EPMC5069574 | biostudies-literature
| S-EPMC7480370 | biostudies-literature
| S-EPMC2546871 | biostudies-literature
| S-EPMC9772203 | biostudies-literature
| S-EPMC5627516 | biostudies-literature
| S-EPMC7754283 | biostudies-literature
| S-EPMC3557752 | biostudies-literature