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Multiplexed Profiling of Single Extracellular Vesicles.

ABSTRACT: Extracellular vesicles (EV) are a family of cell-originating, membrane-enveloped nanoparticles with diverse biological function, diagnostic potential, and therapeutic applications. While EV can be abundant in circulation, their small size (?4 order of magnitude smaller than cells) has necessitated bulk analyses, making many more nuanced biological explorations, cell of origin questions, or heterogeneity investigations impossible. Here we describe a single EV analysis (SEA) technique which is simple, sensitive, multiplexable, and practical. We profiled glioblastoma EV and discovered surprising variations in putative pan-EV as well as tumor cell markers on EV. These analyses shed light on the heterogeneous biomarker profiles of EV. The SEA technology has the potential to address fundamental questions in vesicle biology and clinical applications.

SUBMITTER: Lee K

PROVIDER: S-EPMC5898240 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

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Multiplexed Profiling of Single Extracellular Vesicles.

Lee Kyungheon K Fraser Kyle K Ghaddar Bassel B Yang Katy K Kim Eunha E Balaj Leonora L Chiocca E Antonio EA Breakefield Xandra O XO Lee Hakho H Weissleder Ralph R

ACS nano 20180104 1

Extracellular vesicles (EV) are a family of cell-originating, membrane-enveloped nanoparticles with diverse biological function, diagnostic potential, and therapeutic applications. While EV can be abundant in circulation, their small size (∼4 order of magnitude smaller than cells) has necessitated bulk analyses, making many more nuanced biological explorations, cell of origin questions, or heterogeneity investigations impossible. Here we describe a single EV analysis (SEA) technique which is sim ...[more]

PMID: 29286635

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