Plasmon-Enhanced Biosensing for Multiplexed Profiling of Extracellular Vesicles.

ABSTRACT: Extracellular vesicles (EVs)-nanoscale phospholipid vesicles secreted by cells-present new opportunities for molecular diagnosis from non-invasive liquid biopsies. Single EV protein analysis can be extremely valuable in studying EVs as circulating cancer biomarkers, but it is technically challenging due to weak detection signals associated with limited amounts of epitopes and small surface areas for antibody labeling. Here, a new, simple method that enables multiplexed analyses of EV markers with improved sensitivities is reported. Specifically, plasmon-enhanced fluorescence detection is implemented that amplifies fluorescence signals using surface plasmon resonances excited by periodic gold nanohole structures. It is shown that fluorescence signals in multiple channels are amplified by on

SUBMITTER: Min J 

PROVIDER: S-EPMC7718316 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature