Dataset Information
A novel real-time RT-PCR assay for influenza C tested in Peruvian children.
Ontology highlight
ABSTRACT: Background
Influenza C virus (ICV) is associated with acute respiratory illness. Yet ICV remains under recognized, with most previous studies using only culture to identify cases.Objectives
To develop a sensitive and specific real-time RT-PCR assay for ICV that allows for rapid and accurate detection in a clinical or research setting.Study design
Multiple ICV sequences obtained from GenBank were analyzed, including 141 hemagglutinin-esterase (HE), 106 matrix (M), and 97 nucleoprotein (NP) sequences. Primers and probes were designed based on conserved regions. Multiple primer-probe sets were tested against multiple ICV strains.Results
The ICV M and NP genes offered the most conserved sequence regions. Primers and probes based on newer sequence data offered enhanced detection of ICV, especially for low titer specimens. An NP-targeted assay yielded the best performance and was capable of detecting 10-100 RNA copies per reaction. The NP assay detected multiple clinical isolates of ICV collected in a field epidemiology study conducted in Peru.Conclusions
We report a new real-time RT-PCR assay for ICV with high sensitivity and specificity.
SUBMITTER: Howard LM
PROVIDER: S-EPMC5901714 | biostudies-literature | 2017 Nov
REPOSITORIES: biostudies-literature
Publications
A novel real-time RT-PCR assay for influenza C tested in Peruvian children.
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology 20170901
<h4>Background</h4>Influenza C virus (ICV) is associated with acute respiratory illness. Yet ICV remains under recognized, with most previous studies using only culture to identify cases.<h4>Objectives</h4>To develop a sensitive and specific real-time RT-PCR assay for ICV that allows for rapid and accurate detection in a clinical or research setting.<h4>Study design</h4>Multiple ICV sequences obtained from GenBank were analyzed, including 141 hemagglutinin-esterase (HE), 106 matrix (M), and 97 n ...[more]