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Cryogenic IR spectroscopy combined with ion mobility spectrometry for the analysis of human milk oligosaccharides.

ABSTRACT: We report here our combination of cryogenic, messenger-tagging, infrared (IR) spectroscopy with ion mobility spectrometry (IMS) and mass spectrometry (MS) as a way to identify and analyze a set of human milk oligosaccharides (HMOs) ranging from trisaccharides to hexasaccharides. The added dimension of IR spectroscopy provides a diagnostic fingerprint in the OH and NH stretching region, which is crucial to identify these oligosaccharides, which are difficult to distinguish by IMS alone. These results extend our previous work in demonstrating the generality of this combined approach for distinguishing subtly different structural and regioisomers of glycans of biologically relevant size.

SUBMITTER: Khanal N

PROVIDER: S-EPMC5902639 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

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Cryogenic IR spectroscopy combined with ion mobility spectrometry for the analysis of human milk oligosaccharides.

Khanal Neelam N Masellis Chiara C Kamrath Michael Z MZ Clemmer David E DE Rizzo Thomas R TR

The Analyst 20180401 8

We report here our combination of cryogenic, messenger-tagging, infrared (IR) spectroscopy with ion mobility spectrometry (IMS) and mass spectrometry (MS) as a way to identify and analyze a set of human milk oligosaccharides (HMOs) ranging from trisaccharides to hexasaccharides. The added dimension of IR spectroscopy provides a diagnostic fingerprint in the OH and NH stretching region, which is crucial to identify these oligosaccharides, which are difficult to distinguish by IMS alone. These res ...[more]

PMID: 29541730

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