Ontology highlight
ABSTRACT:
SUBMITTER: Kang JY
PROVIDER: S-EPMC5903582 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Molecular cell 20180301 5
Sequence-specific pausing by RNA polymerase (RNAP) during transcription plays crucial and diverse roles in gene expression. In bacteria, RNA structures are thought to fold within the RNA exit channel of the RNAP and can increase pause lifetimes significantly. The biophysical mechanism of pausing is uncertain. We used single-particle cryo-EM to determine structures of paused complexes, including a 3.8-Å structure of an RNA hairpin-stabilized, paused RNAP that coordinates RNA folding in the his op ...[more]