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Dual-color metal-induced and Forster resonance energy transfer for cell nanoscopy.

ABSTRACT: We report a novel method, dual-color axial nanometric localization by metal--induced energy transfer, and combine it with Förster resonance energy transfer (FRET) for resolving structural details in cells on the molecular level. We demonstrate the capability of this method on cytoskeletal elements and adhesions in human mesenchymal stem cells. Our approach is based on fluorescence-lifetime-imaging microscopy and allows for precise determination of the three-dimensional architecture of stress fibers anchoring at focal adhesions, thus yielding crucial information to understand cell-matrix mechanics. In addition to resolving nanometric structural details along the z-axis, we use FRET to gain precise information on the distance between actin and vinculin at focal adhesions.

SUBMITTER: Chizhik AM

PROVIDER: S-EPMC5905297 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

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Dual-color metal-induced and Förster resonance energy transfer for cell nanoscopy.

Chizhik Anna M AM Wollnik Carina C Ruhlandt Daja D Karedla Narain N Chizhik Alexey I AI Hauke Lara L Hähnel Dirk D Gregor Ingo I Enderlein Jörg J Rehfeldt Florian F

Molecular biology of the cell 20180401 7

We report a novel method, dual-color axial nanometric localization by metal--induced energy transfer, and combine it with Förster resonance energy transfer (FRET) for resolving structural details in cells on the molecular level. We demonstrate the capability of this method on cytoskeletal elements and adhesions in human mesenchymal stem cells. Our approach is based on fluorescence-lifetime-imaging microscopy and allows for precise determination of the three-dimensional architecture of stress fib ...[more]

PMID: 29444956

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