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Interferometric temporal focusing microscopy using three-photon excitation fluorescence.

ABSTRACT: Super-resolution microscopy has become a powerful tool for biological research. However, its spatial resolution and imaging depth are limited, largely due to background light. Interferometric temporal focusing (ITF) microscopy, which combines structured illumination microscopy and three-photon excitation fluorescence microscopy, can overcome these limitations. Here, we demonstrate ITF microscopy using three-photon excitation fluorescence, which has a spatial resolution of 106 nm at an imaging depth of 100 µm with an excitation wavelength of 1060 nm.

SUBMITTER: Toda K

PROVIDER: S-EPMC5905902 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

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Interferometric temporal focusing microscopy using three-photon excitation fluorescence.

Toda Keisuke K Isobe Keisuke K Namiki Kana K Kawano Hiroyuki H Miyawaki Atsushi A Midorikawa Katsumi K

Biomedical optics express 20180306 4

Super-resolution microscopy has become a powerful tool for biological research. However, its spatial resolution and imaging depth are limited, largely due to background light. Interferometric temporal focusing (ITF) microscopy, which combines structured illumination microscopy and three-photon excitation fluorescence microscopy, can overcome these limitations. Here, we demonstrate ITF microscopy using three-photon excitation fluorescence, which has a spatial resolution of 106 nm at an imaging de ...[more]

PMID: 29675298

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