Dataset Information

A study on fast calling variants from next-generation sequencing data using decision tree.

ABSTRACT: BACKGROUND:The rapid development of next-generation sequencing (NGS) technology has continuously been refreshing the throughput of sequencing data. However, due to the lack of a smart tool that is both fast and accurate, the analysis task for NGS data, especially those with low-coverage, remains challenging. RESULTS:We proposed a decision-tree based variant calling algorithm. Experiments on a set of real data indicate that our algorithm achieves high accuracy and sensitivity for SNVs and indels and shows good adaptability on low-coverage data. In particular, our algorithm is obviously faster than 3 widely used tools in our experiments. CONCLUSIONS:We implemented our algorithm in a software named Fuwa and applied it together with 4 well-known variant callers, i.e., Platypus, GATK-UnifiedGenotyper, GATK-HaplotypeCaller and SAMtools, to three sequencing data sets of a well-studied sample NA12878, which were produced by whole-genome, whole-exome and low-coverage whole-genome sequencing technology respectively. We also conducted additional experiments on the WGS data of 4 newly released samples that have not been used to populate dbSNP.

SUBMITTER: Li Z

PROVIDER: S-EPMC5907718 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

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A study on fast calling variants from next-generation sequencing data using decision tree.

Li Zhentang Z Wang Yi Y Wang Fei F

BMC bioinformatics 20180419 1

<h4>Background</h4>The rapid development of next-generation sequencing (NGS) technology has continuously been refreshing the throughput of sequencing data. However, due to the lack of a smart tool that is both fast and accurate, the analysis task for NGS data, especially those with low-coverage, remains challenging.<h4>Results</h4>We proposed a decision-tree based variant calling algorithm. Experiments on a set of real data indicate that our algorithm achieves high accuracy and sensitivity for S ...[more]

PMID: 29673316

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Project description:Insertions/deletions (indels) are the second most common type of genomic variant and the most common type of structural variant. Identification of indels in next generation sequencing data is a challenge, and algorithms commonly used for indel detection have not been compared on a research cohort of human subject genomic data. Guidelines for the optimal detection of biologically significant indels are limited. We analyzed three sets of human next generation sequencing data (48 samples of a 200 gene target exon sequencing, 45 samples of whole exome sequencing, and 2 samples of whole genome sequencing) using three algorithms for indel detection (Pindel, Genome Analysis Tool Kit's UnifiedGenotyper and HaplotypeCaller).We observed variation in indel calls across the three algorithms. The intersection of the three tools comprised only 5.70% of targeted exon, 19.52% of whole exome, and 14.25% of whole genome indel calls. The majority of the discordant indels were of lower read depth and likely to be false positives. When software parameters were kept consistent across the three targets, HaplotypeCaller produced the most reliable results. Pindel results did not validate well without adjustments to parameters to account for varied read depth and number of samples per run. Adjustments to Pindel's M (minimum support for event) parameter improved both concordance and validation rates. Pindel was able to identify large deletions that surpassed the length capabilities of the GATK algorithms.Despite the observed variability in indel identification, we discerned strengths among the individual algorithms on specific data sets. This allowed us to suggest best practices for indel calling. Pindel's low validation rate of indel calls made in targeted exon sequencing suggests that HaplotypeCaller is better suited for short indels and multi-sample runs in targets with very high read depth. Pindel allows for optimization of minimum support for events and is best used for detection of larger indels at lower read depths.

Dataset Information

A study on fast calling variants from next-generation sequencing data using decision tree.

Publications

A study on fast calling variants from next-generation sequencing data using decision tree.

Similar Datasets

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