Ontology highlight
ABSTRACT:
SUBMITTER: O'Connell GC
PROVIDER: S-EPMC5907901 | biostudies-literature | 2017 Nov
REPOSITORIES: biostudies-literature
O'Connell Grant C GC Treadway Madison B MB Petrone Ashley B AB Tennant Connie S CS Lucke-Wold Noelle N Chantler Paul D PD Barr Taura L TL
Laboratory medicine 20171101 4
<h4>Background</h4>The use of reference genes for normalization of whole blood qRT-PCR data may be problematic in conditions such as stroke which induce alterations in white blood cell differential. In this study, we assessed the influence of stroke on the stability of commonly employed reference genes, and we evaluated data-driven normalization as an alternative.<h4>Methods</h4>Peripheral whole blood was sampled from 33 stroke patients and 29 controls, and qRT-PCR was used to measure the expres ...[more]