Ontology highlight
ABSTRACT:
SUBMITTER: Limsakul P
PROVIDER: S-EPMC5910193 | biostudies-literature | 2018 Apr
REPOSITORIES: biostudies-literature
Limsakul Praopim P Peng Qin Q Wu Yiqian Y Allen Molly E ME Liang Jing J Remacle Albert G AG Lopez Tyler T Ge Xin X Kay Brian K BK Zhao Huimin H Strongin Alex Y AY Yang Xiang-Lei XL Lu Shaoying S Wang Yingxiao Y
Cell chemical biology 20180127 4
Monitoring enzymatic activities at the cell surface is challenging due to the poor efficiency of transport and membrane integration of fluorescence resonance energy transfer (FRET)-based biosensors. Therefore, we developed a hybrid biosensor with separate donor and acceptor that assemble in situ. The directed evolution and sequence-function analysis technologies were integrated to engineer a monobody variant (PEbody) that binds to R-phycoerythrin (R-PE) dye. PEbody was used for visualizing the d ...[more]