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Label-free and live cell imaging by interferometric scattering microscopy.

ABSTRACT: Despite recent remarkable advances in microscopic techniques, it still remains very challenging to directly observe the complex structure of cytoplasmic organelles in live cells without a fluorescent label. Here we report label-free and live-cell imaging of mammalian cell, Escherischia coli, and yeast, using interferometric scattering microscopy, which reveals the underlying structures of a variety of cytoplasmic organelles as well as the underside structure of the cells. The contact areas of the cells attached onto a glass substrate, e.g., focal adhesions and filopodia, are clearly discernible. We also found a variety of fringe-like features in the cytoplasmic area, which may reflect the folded structures of cytoplasmic organelles. We thus anticipate that the label-free interferometric scattering microscopy can be used as a powerful tool to shed interferometric light on in vivo structures and dynamics of various intracellular phenomena.

SUBMITTER: Park JS

PROVIDER: S-EPMC5914294 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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Label-free and live cell imaging by interferometric scattering microscopy.

Park Jin-Sung JS Lee Il-Buem IB Moon Hyeon-Min HM Joo Jong-Hyeon JH Kim Kyoung-Hoon KH Hong Seok-Cheol SC Cho Minhaeng M

Chemical science 20180209 10

Despite recent remarkable advances in microscopic techniques, it still remains very challenging to directly observe the complex structure of cytoplasmic organelles in live cells without a fluorescent label. Here we report label-free and live-cell imaging of mammalian cell, <i>Escherischia coli</i>, and yeast, using interferometric scattering microscopy, which reveals the underlying structures of a variety of cytoplasmic organelles as well as the underside structure of the cells. The contact area ...[more]

PMID: 29732052

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