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Investigating Protein-Ligand Interactions by Solution Nuclear Magnetic Resonance Spectroscopy.

ABSTRACT: Protein-ligand interactions are of fundamental importance in almost all processes in living organisms. The ligands comprise small molecules, drugs or biological macromolecules and their interaction strength varies over several orders of magnitude. Solution NMR spectroscopy offers a large repertoire of techniques to study such complexes. Here, we give an overview of the different NMR approaches available. The information they provide ranges from the simple information about the presence of binding or epitope mapping to the complete 3?D structure of the complex. NMR spectroscopy is particularly useful for the study of weak interactions and for the screening of binding ligands with atomic resolution.

SUBMITTER: Becker W

PROVIDER: S-EPMC5915746 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

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Investigating Protein-Ligand Interactions by Solution Nuclear Magnetic Resonance Spectroscopy.

Becker Walter W Bhattiprolu Krishna Chaitanya KC Gubensäk Nina N Zangger Klaus K

Chemphyschem : a European journal of chemical physics and physical chemistry 20180216 8

Protein-ligand interactions are of fundamental importance in almost all processes in living organisms. The ligands comprise small molecules, drugs or biological macromolecules and their interaction strength varies over several orders of magnitude. Solution NMR spectroscopy offers a large repertoire of techniques to study such complexes. Here, we give an overview of the different NMR approaches available. The information they provide ranges from the simple information about the presence of bindin ...[more]

PMID: 29314603

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