Clot stability as a determinant of effective factor VIII replacement in hemophilia A.

ABSTRACT:

Background

Factor VIII (FVIII) replacement is standard of care for patients with hemophilia A (HemA); however, patient response does not always correlate with FVIII levels. We hypothesize this may be in part due to the physical properties of clots and contributions of fibrin, platelets, and erythrocytes, which may be important for hemostasis.

Objective

To understand how FVIII contributes to effective hemostasis in terms of clot structure and mechanical properties.

Patients/methods

In vitro HemA clots in human plasma or whole blood were analyzed using turbidity waveform analysis, confocal microscopy, and rheometry with or without added FVIII. In vivo clots from saphenous vein puncture in wild-type and HemA mice with varying FVIII levels were examined using scanning ele

SUBMITTER: Leong L 

PROVIDER: S-EPMC5920517 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature