Structure of the ACF7 EF-Hand-GAR Module and Delineation of Microtubule Binding Determinants.
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ABSTRACT:
SUBMITTER: Lane TR
PROVIDER: S-EPMC5920566 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
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Structure of the ACF7 EF-Hand-GAR Module and Delineation of Microtubule Binding Determinants.
Lane Thomas R TR Fuchs Elaine E Slep Kevin C KC
Structure (London, England : 1993) 20170608 7
Spectraplakins are large molecules that cross-link F-actin and microtubules (MTs). Mutations in spectraplakins yield defective cell polarization, aberrant focal adhesion dynamics, and dystonia. We present the 2.8 Å crystal structure of the hACF7 EF1-EF2-GAR MT-binding module and delineate the GAR residues critical for MT binding. The EF1-EF2 and GAR domains are autonomous domains connected by a flexible linker. The EF1-EF2 domain is an EFβ-scaffold with two bound Ca<sup>2+</sup> ions that stradd ...[more]
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