Ontology highlight
ABSTRACT:
SUBMITTER: Staller MV
PROVIDER: S-EPMC5920710 | biostudies-literature | 2018 Apr
REPOSITORIES: biostudies-literature
Staller Max V MV Holehouse Alex S AS Swain-Lenz Devjanee D Das Rahul K RK Pappu Rohit V RV Cohen Barak A BA
Cell systems 20180307 4
Transcriptional activation domains are essential for gene regulation, but their intrinsic disorder and low primary sequence conservation have made it difficult to identify the amino acid composition features that underlie their activity. Here, we describe a rational mutagenesis scheme that deconvolves the function of four activation domain sequence features-acidity, hydrophobicity, intrinsic disorder, and short linear motifs-by quantifying the activity of thousands of variants in vivo and simula ...[more]