Ontology highlight
ABSTRACT:
SUBMITTER: Chan WC
PROVIDER: S-EPMC5923034 | biostudies-literature | 2017 Oct
REPOSITORIES: biostudies-literature
Chembiochem : a European journal of chemical biology 20170823 19
Methods for activating signaling enzymes hold significant potential for the study of cellular signal transduction. Here we present a strategy for engineering chemically activatable protein tyrosine phosphatases (actPTPs). To generate actPTP1B, we introduced three cysteine point mutations in the enzyme's WPD loop. Biarsenical compounds were screened for the capability to bind actPTP1B's WPD loop and increase its phosphatase activity. We identified AsCy3-EDT<sub>2</sub> as a robust activator that ...[more]