Ontology highlight
ABSTRACT:
SUBMITTER: Dong Q
PROVIDER: S-EPMC5924926 | biostudies-literature | 2018 Apr
REPOSITORIES: biostudies-literature
Dong Qiang Q Li Xiang X Wang Cheng-Zhi CZ Xu Shaohua S Yuan Gang G Shao Wei W Liu Baodong B Zheng Yong Y Wang Hailin H Lei Xiaoguang X Zhang Zhuqiang Z Zhu Bing B
Proceedings of the National Academy of Sciences of the United States of America 20180410 17
Epigenetic silencing can be mediated by various mechanisms, and many regulators remain to be identified. Here, we report a genome-wide siRNA screening to identify regulators essential for maintaining gene repression of a CMV promoter silenced by DNA methylation. We identified CSE1L (chromosome segregation 1 like) as an essential factor for the silencing of the reporter gene and many endogenous methylated genes. CSE1L depletion did not cause DNA demethylation. On the other hand, the methylated ge ...[more]