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Sample manipulation and data assembly for robust microcrystal synchrotron crystallography.

ABSTRACT: With the recent developments in microcrystal handling, synchrotron microdiffraction beamline instrumentation and data analysis, microcrystal crystallo-graphy with crystal sizes of less than 10?µm is appealing at synchrotrons. However, challenges remain in sample manipulation and data assembly for robust microcrystal synchrotron crystallography. Here, the development of micro-sized polyimide well-mounts for the manipulation of microcrystals of a few micrometres in size and the implementation of a robust data-analysis method for the assembly of rotational microdiffraction data sets from many microcrystals are described. The method demonstrates that microcrystals may be routinely utilized for the acquisition and assembly of complete data sets from synchrotron microdiffraction beamlines.

SUBMITTER: Guo G

PROVIDER: S-EPMC5929371 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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Sample manipulation and data assembly for robust microcrystal synchrotron crystallography.

Guo Gongrui G Fuchs Martin R MR Shi Wuxian W Skinner John J Berman Evanna E Ogata Craig M CM Hendrickson Wayne A WA McSweeney Sean S Liu Qun Q

IUCrJ 20180419 Pt 3

With the recent developments in microcrystal handling, synchrotron microdiffraction beamline instrumentation and data analysis, microcrystal crystallo-graphy with crystal sizes of less than 10 µm is appealing at synchrotrons. However, challenges remain in sample manipulation and data assembly for robust microcrystal synchrotron crystallography. Here, the development of micro-sized polyimide well-mounts for the manipulation of microcrystals of a few micrometres in size and the implementation of a ...[more]

PMID: 29755741

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