The Effects of Ultraviolet Radiation on Nucleoside Modifications in RNA.
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ABSTRACT:
SUBMITTER: Sun C
PROVIDER: S-EPMC5933051 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Publications
The Effects of Ultraviolet Radiation on Nucleoside Modifications in RNA.
Sun Congliang C Jora Manasses M Solivio Beulah B Limbach Patrick A PA Addepalli Balasubrahmanyam B
ACS chemical biology 20180205 3
Ultraviolet radiation (UVR) is a known genotoxic agent. Although its effects on DNA have been well-documented, its impact on RNA and RNA modifications is less studied. By using Escherichia coli tRNA (tRNA) as a model system, we identify the UVA (370 nm) susceptible chemical groups and bonds in a large variety of modified nucleosides. We use liquid chromatography tandem mass spectrometry to identify specific nucleoside photoproducts under in vitro and in vivo conditions, which were then verified ...[more]
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