IPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications.

ABSTRACT: Hibernating mammals survive hypothermia (<10°C) without injury, a remarkable feat of cellular preservation that bears significance for potential medical applications. However, mechanisms imparting cold resistance, such as cytoskeleton stability, remain elusive. Using the first iPSC line from a hibernating mammal (13-lined ground squirrel), we uncovered cellular pathways critical for cold tolerance. Comparison between human and ground squirrel iPSC-derived neurons revealed differential mitochondrial and protein quality control responses to cold. In human iPSC-neurons, cold triggered mitochondrial stress, resulting in reactive oxygen species overproduction and lysosomal membrane permeabilization, contributing to microtubule destruction. Manipulations of these pathways endowed microtubule col

SUBMITTER: Ou J 

PROVIDER: S-EPMC5935596 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature