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Pressurized CO₂ as a carboxylating agent for the biocatalytic ortho-carboxylation of resorcinol.

ABSTRACT: The utilization of gaseous carbon dioxide instead of bicarbonate would greatly facilitate process development for enzyme catalyzed carboxylations on a large scale. As a proof-of-concept, 1,3-dihydroxybenzene (resorcinol) was carboxylated in the ortho-position using pressurized CO₂ (?30-40 bar) catalyzed by ortho-benzoic acid decarboxylases with up to 68% conversion. Optimization studies revealed tight pH-control and enzyme stability as the most important determinants.

SUBMITTER: Plasch K

PROVIDER: S-EPMC5942041 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

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Pressurized CO2 as a carboxylating agent for the biocatalytic ortho-carboxylation of resorcinol.

Plasch Katharina K Hofer Gerhard G Keller Walter W Hay Sam S Heyes Derren J DJ Dennig Alexander A Glueck Silvia M SM Faber Kurt K

Green chemistry : an international journal and green chemistry resource : GC 20180403 8

The utilization of gaseous carbon dioxide instead of bicarbonate would greatly facilitate process development for enzyme catalyzed carboxylations on a large scale. As a proof-of-concept, 1,3-dihydroxybenzene (resorcinol) was carboxylated in the ortho-position using pressurized CO2 (∼30-40 bar) catalyzed by ortho-benzoic acid decarboxylases with up to 68% conversion. Optimization studies revealed tight pH-control and enzyme stability as the most important determinants. ...[more]

PMID: 29780282

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