MicroRNA 21a-5p overexpression impacts mediators of extracellular matrix formation in uterine leiomyoma.

ABSTRACT:

Background

MicroRNAs (MiR) may promote fibroid development via altered expression of genes involved in cell proliferation and ECM formation, and evidence supports aberrant expression of MicroRNA (MiR) 21a-5p in fibroids. The purpose of this study was to investigate the functional significance of MiR 21a-5p overexpression in the pathobiology of leiomyomata (fibroids).

Methods

A basic science experimental design using immortalized fibroid and myometrial cell lines derived from patient-matched specimens was used. Stable overexpression of MiR-21a-5p in an immortalized fibroid and patient matched myometrial cell line was achieved through lentiviral vector infection. Main outcome measures were MiR-21-5p overexpression, target gene and protein expression, collagen (COL1A1) producti

SUBMITTER: Cardozo ER 

PROVIDER: S-EPMC5946472 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature