Ontology highlight
ABSTRACT:
SUBMITTER: Merkle PS
PROVIDER: S-EPMC5947982 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Merkle Patrick S PS Gotfryd Kamil K Cuendet Michel A MA Leth-Espensen Katrine Z KZ Gether Ulrik U Loland Claus J CJ Rand Kasper D KD
Science advances 20180511 5
LeuT, a prokaryotic member of the neurotransmitter:sodium symporter (NSS) family, is an established structural model for mammalian NSS counterparts. We investigate the substrate translocation mechanism of LeuT by measuring the solution-phase structural dynamics of the transporter in distinct functional states by hydrogen/deuterium exchange mass spectrometry (HDX-MS). Our HDX-MS data pinpoint LeuT segments involved in substrate transport and reveal for the first time a comprehensive and detailed ...[more]