Ontology highlight
ABSTRACT:
SUBMITTER: Kamikawa Y
PROVIDER: S-EPMC5952543 | biostudies-literature | 2016 Jan
REPOSITORIES: biostudies-literature
Chemical science 20150930 1
Conditional fluorescence imaging is a powerful technique for precise spatiotemporal analysis of proteins in live cells upon administration of a synthetic probe. To be applicable to various biological phenomena, probes must be membrane-permeable, have a high signal-to-noise ratio, and work quickly. To date, few probes meet all of these requirements. Here, we designed a fluorogenic probe (AcFCANB) that could label intracellular proteins fused to the photoactive yellow protein (PYP) tag in live cel ...[more]