Measurement of Mesoscale Conformational Dynamics of Freely Diffusing Molecules with Tracking FCS.

ABSTRACT: Few techniques are suited to probe the structure and dynamics of molecular complexes at the mesoscale level (?100-1000 nm). We have developed a single-molecule technique that uses tracking fluorescence correlation spectroscopy (tFCS) to probe the conformation and dynamics of mesoscale molecular assemblies. tFCS measures the distance fluctuations between two fluorescently labeled sites within an untethered, freely diffusing biomolecule. To achieve subdiffraction spatial resolution, we developed a feedback scheme that allows us to maintain the molecule at an optimal position within the laser intensity gradient for fluorescence correlation spectroscopy. We characterized tFCS spatial sensitivity by measuring the Brownian end-to-end dynamics of DNA molecules as short as 1000 bp. We demonstrate that tFCS detects changes in the compaction of reconstituted nucleosome arrays and can assay transient protein-mediated interactions between distant sites in an individual DNA molecule. Our measurements highlight the applicability of tFCS to a wide variety of biochemical processes involving mesoscale conformational dynamics.

SUBMITTER: Limouse C 

PROVIDER: S-EPMC5954409 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature