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Cloning and Differential Expression Analyses of Cdc42 from Sheep.

ABSTRACT: Introduction:Serological diagnosis of brucellosis is still a great challenge due to the infeasibility of discriminating infected animals from vaccinated ones, so it is necessary to search for diagnostic biomarkers for differential diagnosis of brucellosis. Material and Methods:Cell division cycle 42 (Cdc42) from sheep (Ovis aries) (OaCdc42) was cloned by rapid amplification of cDNA ends (RACE), and then tissue distribution and differential expression levels of OaCdc42 mRNA between infected and vaccinated sheep were analysed by RT-qPCR. Results:The full-length cDNA of OaCdc42 was 1,609 bp containing an open reading frame (ORF) of 576 bp. OaCdc42 mRNAs were detected in the heart, liver, spleen, lung, kidneys, rumen, small intestine, skeletal muscles, and buffy coat, and the highest expression was detected in the small intestine. Compared to the control, the levels of OaCdc42 mRNA from sheep infected with Brucella melitensis or sheep vaccinated with Brucella suis S2 was significantly different (P < 0.01) after 40 and 30 days post-inoculation, respectively. However, the expression of OaCdc42 mRNA was significantly different between vaccinated and infected sheep (P < 0.05 or P < 0.01) on days: 14, 30, and 60 post-inoculation, whereas no significant difference (P > 0.05) was noted 40 days post-inoculation. Moreover, the expression of OaCdc42 from both infected and vaccinated sheep showed irregularity. Conclusion:OaCdc42 is not a good potential diagnostic biomarker for differential diagnosis of brucellosis in sheep.

SUBMITTER: Yang YJ

PROVIDER: S-EPMC5957470 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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Cloning and Differential Expression Analyses of Cdc42 from Sheep.

Yang Yong-Jie YJ Liu Zeng-Shan ZS Lu Shi-Ying SY Hu Pan P Li Chuang C Ahmad Waqas W Li Yan-Song YS Xu Yun-Ming YM Tang Feng F Zhou Yu Y Ren Hong-Lin HL

Journal of veterinary research 20180330 1

<h4>Introduction</h4>Serological diagnosis of brucellosis is still a great challenge due to the infeasibility of discriminating infected animals from vaccinated ones, so it is necessary to search for diagnostic biomarkers for differential diagnosis of brucellosis.<h4>Material and methods</h4>Cell division cycle 42 (Cdc42) from sheep (<i>Ovis aries</i>) (OaCdc42) was cloned by rapid amplification of cDNA ends (RACE), and then tissue distribution and differential expression levels of OaCdc42 mRNA ...[more]

PMID: 29978136

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Dataset Information

Cloning and Differential Expression Analyses of Cdc42 from Sheep.

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Cloning and Differential Expression Analyses of Cdc42 from Sheep.

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