Ontology highlight
ABSTRACT:
SUBMITTER: Sigmund F
PROVIDER: S-EPMC5959871 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Sigmund Felix F Massner Christoph C Erdmann Philipp P Stelzl Anja A Rolbieski Hannes H Desai Mitul M Bricault Sarah S Wörner Tobias P TP Snijder Joost J Geerlof Arie A Fuchs Helmut H Hrabĕ de Angelis Martin M Heck Albert J R AJR Jasanoff Alan A Ntziachristos Vasilis V Plitzko Jürgen J Westmeyer Gil G GG
Nature communications 20180518 1
We genetically controlled compartmentalization in eukaryotic cells by heterologous expression of bacterial encapsulin shell and cargo proteins to engineer enclosed enzymatic reactions and size-constrained metal biomineralization. The shell protein (EncA) from Myxococcus xanthus auto-assembles into nanocompartments inside mammalian cells to which sets of native (EncB,C,D) and engineered cargo proteins self-target enabling localized bimolecular fluorescence and enzyme complementation. Encapsulatio ...[more]