Dataset Information

Tissue distribution of oral vitamin B12 is influenced by B12 status and B12 form: an experimental study in rats.

ABSTRACT:

Purpose

Hydroxocobalamin (HOCbl) is the dominating Cbl form in food, whereas cyanocobalamin (CNCbl) is common in vitamin pills and oral supplements. This study compares single-dose absorption and distribution of oral HO[⁵⁷Co]Cbl and CN[⁵⁷Co]Cbl in Cbl-deficient and normal rats.

Methods

Male Wistar rats (7 weeks) were fed a 14-day diet with (n?=?15) or without (n?=?15) Cbl. We compared the uptakes of HO[⁵⁷Co]Cbl (free or bound to bovine transcobalamin) and free CN[⁵⁷Co]Cbl administered by gastric gavage (n?=?5 in each diet group). Rats were sacrificed after 24 h. Blood, liver, kidney, brain, heart, spleen, intestines, skeletal muscle, 24-h urine and faeces were collected, and the content of [⁵⁷Co]Cbl was measured. Endogenous Cbl in tissues and plasma was analysed by routine methods.

Results

Mean endogenous plasma-Cbl was sevenfold lower in deficient vs. normal rats (190 vs. 1330 pmol/L, p?in/k _out) in all organs except for the kidney, where the ratio decreased considerably. Twenty-four-hour accumulation of labelled Cbl showed that HOCbl?>?CNCbl (liver) and CNCbl?>?HOCbl (brain, muscle and plasma).

Conclusions

The Cbl status of rats and the administered Cbl form influence 24-h Cbl accumulation in tissues and plasma.

SUBMITTER: Kornerup LS

PROVIDER: S-EPMC5960002 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Publications

Tissue distribution of oral vitamin B12 is influenced by B12 status and B12 form: an experimental study in rats.

Kornerup Linda S LS Fedosov Sergey N SN Juul Christian B CB Greibe Eva E Heegaard Christian W CW Nexo Ebba E

European journal of nutrition 20170320 4

<h4>Purpose</h4>Hydroxocobalamin (HOCbl) is the dominating Cbl form in food, whereas cyanocobalamin (CNCbl) is common in vitamin pills and oral supplements. This study compares single-dose absorption and distribution of oral HO[<sup>57</sup>Co]Cbl and CN[<sup>57</sup>Co]Cbl in Cbl-deficient and normal rats.<h4>Methods</h4>Male Wistar rats (7 weeks) were fed a 14-day diet with (n = 15) or without (n = 15) Cbl. We compared the uptakes of HO[<sup>57</sup>Co]Cbl (free or bound to bovine transcobalam ...[more]

PMID: 28321545

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Project description:BackgroundVitamin B12 is an essential micronutrient in humans needed for health maintenance. Deficiency of vitamin B12 has been linked to dietary, environmental and genetic factors. Evidence for the genetic basis of vitamin B12 status is poorly understood. However, advancements in genomic techniques have increased the knowledge-base of the genetics of vitamin B12 status. Based on the candidate gene and genome-wide association (GWA) studies, associations between genetic loci in several genes involved in vitamin B12 metabolism have been identified.ObjectiveThe objective of this literature review was to identify and discuss reports of associations between single-nucleotide polymorphisms (SNPs) in vitamin B12 pathway genes and their influence on the circulating levels of vitamin B12.MethodsRelevant articles were obtained through a literature search on PubMed through to May 2017. An article was included if it examined an association of a SNP with serum or plasma vitamin B12 concentration. Beta coefficients and odds ratios were used to describe the strength of an association, and a P < 0.05 was considered as statistically significant. Two reviewers independently evaluated the eligibility for the inclusion criteria and extracted the data.ResultsFrom 23 studies which fulfilled the selection criteria, 16 studies identified SNPs that showed statistically significant associations with vitamin B12 concentrations. Fifty-nine vitamin B12-related gene polymorphisms associated with vitamin B12 status were identified in total, from the following populations: African American, Brazilian, Canadian, Chinese, Danish, English, European ancestry, Icelandic, Indian, Italian, Latino, Northern Irish, Portuguese and residents of the USA.ConclusionOverall, the data analyzed suggests that ethnic-specific associations are involved in the genetic determination of vitamin B12 concentrations. However, despite recent success in genetic studies, the majority of identified genes that could explain variation in vitamin B12 concentrations were from Caucasian populations. Further research utilizing larger sample sizes of non-Caucasian populations is necessary in order to better understand these ethnic-specific associations.

Dataset Information

Tissue distribution of oral vitamin B12 is influenced by B12 status and B12 form: an experimental study in rats.

Purpose

Methods

Results

Conclusions

Publications

Tissue distribution of oral vitamin B12 is influenced by B12 status and B12 form: an experimental study in rats.

Similar Datasets

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