Simultaneous cell disruption and semi-quantitative activity assays for high-throughput screening of thermostable L-asparaginases.
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ABSTRACT:
SUBMITTER: Li X
PROVIDER: S-EPMC5962637 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Publications
Simultaneous cell disruption and semi-quantitative activity assays for high-throughput screening of thermostable L-asparaginases.
Li Xu X Zhang Xian X Xu Shuqin S Zhang Hengwei H Xu Meijuan M Yang Taowei T Wang Li L Qian Haifeng H Zhang Huiling H Fang Haitian H Osire Tolbert T Rao Zhiming Z Yang Shangtian S
Scientific reports 20180521 1
L-asparaginase, which catalyses the hydrolysis of L-asparagine to L-aspartate, has attracted the attention of researchers due to its expanded applications in medicine and the food industry. In this study, a novel thermostable L-asparaginase from Pyrococcus yayanosii CH1 was cloned and over-expressed in Bacillus subtilis 168. To obtain thermostable L-asparaginase mutants with higher activity, a robust high-throughput screening process was developed specifically for thermophilic enzymes. In this p ...[more]
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