Ontology highlight
ABSTRACT:
SUBMITTER: Krastev DB
PROVIDER: S-EPMC5964205 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Krastev Dragomir B DB Pettitt Stephen J SJ Campbell James J Song Feifei F Tanos Barbara E BE Stoynov Stoyno S SS Ashworth Alan A Lord Christopher J CJ
Nature communications 20180522 1
Poly (ADP-ribose)ylation is a dynamic protein modification that regulates multiple cellular processes. Here, we describe a system for identifying and characterizing PARylation events that exploits the ability of a PBZ (PAR-binding zinc finger) protein domain to bind PAR with high-affinity. By linking PBZ domains to bimolecular fluorescent complementation biosensors, we developed fluorescent PAR biosensors that allow the detection of temporal and spatial PARylation events in live cells. Exploitin ...[more]