Ontology highlight
ABSTRACT:
SUBMITTER: Panigrahi AK
PROVIDER: S-EPMC5966282 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Panigrahi Anil K AK Foulds Charles E CE Lanz Rainer B RB Hamilton Ross A RA Yi Ping P Lonard David M DM Tsai Ming-Jer MJ Tsai Sophia Y SY O'Malley Bert W BW
Molecular cell 20180501 4
Enhancers are thought to activate transcription by physically contacting promoters via looping. However, direct assays demonstrating these contacts are required to mechanistically verify such cellular determinants of enhancer function. Here, we present versatile cell-free assays to further determine the role of enhancer-promoter contacts (EPCs). We demonstrate that EPC is linked to mutually stimulatory transcription at the enhancer and promoter in vitro. SRC-3 was identified as a critical loopin ...[more]