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An antibacterial platform based on capacitive carbon-doped TiO2 nanotubes after direct or alternating current charging.

ABSTRACT: Electrical interactions between bacteria and the environment are delicate and essential. In this study, an external electrical current is applied to capacitive titania nanotubes doped with carbon (TNT-C) to evaluate the effects on bacteria killing and the underlying mechanism is investigated. When TNT-C is charged, post-charging antibacterial effects proportional to the capacitance are observed. This capacitance-based antibacterial system works well with both direct and alternating current (DC, AC) and the higher discharging capacity in the positive DC (DC+) group leads to better antibacterial performance. Extracellular electron transfer observed during early contact contributes to the surface-dependent post-charging antibacterial process. Physiologically, the electrical interaction deforms the bacteria morphology and elevates the intracellular reactive oxygen species level without impairing the growth of osteoblasts. Our finding spurs the design of light-independent antibacterial materials and provides insights into the use of electricity to modify biomaterials to complement other bacteria killing measures such as light irradiation.

SUBMITTER: Wang G

PROVIDER: S-EPMC5967314 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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An antibacterial platform based on capacitive carbon-doped TiO<sub>2</sub> nanotubes after direct or alternating current charging.

Wang Guomin G Feng Hongqing H Hu Liangsheng L Jin Weihong W Hao Qi Q Gao Ang A Peng Xiang X Li Wan W Wong Kwok-Yin KY Wang Huaiyu H Li Zhou Z Chu Paul K PK

Nature communications 20180524 1

Electrical interactions between bacteria and the environment are delicate and essential. In this study, an external electrical current is applied to capacitive titania nanotubes doped with carbon (TNT-C) to evaluate the effects on bacteria killing and the underlying mechanism is investigated. When TNT-C is charged, post-charging antibacterial effects proportional to the capacitance are observed. This capacitance-based antibacterial system works well with both direct and alternating current (DC, ...[more]

PMID: 29795383

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Project description:One of the major challenges of implantology is to design nanoscale modifications of titanium implant surfaces inducing osseointegration. The aim of this study was to investigate the behavior of rat osteoblasts cultured on anodized TiO2 nanotubes of different crystallinity (amorphous and anatase phase) up to 24 days. TiO2 nanotubes were fabricated on VT1-0 titanium foil via a two-step anodization at 20 V using NH4F as an electrolyte. Anatase-phase samples were prepared by heat treatment at 500 °C for 1 h. VT1-0 samples with flat surfaces were used as controls. Primary rat osteoblasts were seeded over experimental surfaces for several incubation times. Scanning electron microscopy (SEM) was used to analyze tested surfaces and cell morphology. Cell adhesion and proliferation were investigated by cell counting. Osteogenic differentiation of cells was evaluated by qPCR of runt-related transcription factor 2 (RUNX2), osteopontin (OPN), integrin binding sialoprotein (IBSP), alkaline phosphatase (ALP) and osteocalcin (OCN). Cell adhesion and proliferation, cell morphology and the expression of osteogenic markers were affected by TiO2 nanotube layered substrates of amorphous and anatase crystallinity. In comparison with flat titanium, along with increased cell adhesion and cell growth a large portion of osteoblasts grown on the both nanostructured surfaces exhibited an osteocyte-like morphology as early as 48 h of culture. Moreover, the expression of all tested osteogenic markers in cells cultured on amorphous and anatase TiO2 nanotubes was upregulated at least at one of the analyzed time points. To summarize, we demonstrated that amorphous and anodized TiO2 layered substrates are highly biocompatible with rat osteoblasts and that the surface modification with about 1500 nm length nanotubes of 35 ± 4 (amorphous phase) and 41 ± 8 nm (anatase phase) in diameter is sufficient to induce their osteogenic differentiation. Such results are significant to the engineering of coating strategies for orthopedic implants aimed to establish a more efficient bone to implant contact and enhance bone repair.

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An antibacterial platform based on capacitive carbon-doped TiO2 nanotubes after direct or alternating current charging.

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An antibacterial platform based on capacitive carbon-doped TiO<sub>2</sub> nanotubes after direct or alternating current charging.

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An antibacterial platform based on capacitive carbon-doped TiO2 nanotubes after direct or alternating current charging.

Publications

An antibacterial platform based on capacitive carbon-doped TiO&lt;sub&gt;2&lt;/sub&gt; nanotubes after direct or alternating current charging.

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OmicsDI is part of the ELIXIR infrastructure

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An antibacterial platform based on capacitive carbon-doped TiO<sub>2</sub> nanotubes after direct or alternating current charging.