Ischemia-Induced DNA Hypermethylation during Kidney Transplant Predicts Chronic Allograft Injury.

ABSTRACT: Background Ischemia during kidney transplant causes chronic allograft injury and adversely affects outcome, but the underlying mechanisms are incompletely understood. In tumors, oxygen shortage reduces the DNA demethylating activity of the ten-11 translocation (TET) enzymes, yielding hypermethylated genomes that promote tumor progression. We investigated whether ischemia similarly induces DNA hypermethylation in kidney transplants and contributes to chronic injury.Methods We profiled genome-wide DNA methylation in three cohorts of brain-dead donor kidney allograft biopsy specimens: a longitudinal cohort with paired biopsy specimens obtained at allograft procurement (preischemia; n=13), after implantation and reperfusion (postischemia; n=13), and at 3 or 12 month

SUBMITTER: Heylen L 

PROVIDER: S-EPMC5967765 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature