Ontology highlight
ABSTRACT:
SUBMITTER: Hegde M
PROVIDER: S-EPMC5969736 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Hegde Mudra M Strand Christine C Hanna Ruth E RE Doench John G JG
PloS one 20180525 5
Many implementations of pooled screens in mammalian cells rely on linking an element of interest to a barcode, with the latter subsequently quantitated by next generation sequencing. However, substantial uncoupling between these paired elements during lentiviral production has been reported, especially as the distance between elements increases. We detail that PCR amplification is another major source of uncoupling, and becomes more pronounced with increased amounts of DNA template molecules and ...[more]