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Identification of a biliverdin geometric isomer by means of HPLC/ESI-MS and NMR spectroscopy. Differentiation of the isomers by using fragmentation "in-source".

ABSTRACT:

Abstract

A commercially available biliverdin sample was analyzed by means of HPLC/ESI-MS and NMR spectroscopy. It was been found that beside the main IX? 5Z,10Z,15Z isomer, the sample contains also the geometric isomer IX? 5Z,10Z,15E. It was also found the isomers behave differentially upon "in-source" fragmentation in negative ion mode (in contrast to the their behavior upon "in-source" fragmentation in positive ion mode and to their behavior upon MS/MS fragmentation in both modes): the relative abundances of deprotonated molecules and fragment ions are significantly different for both isomers, which can be used as an analytical tool to differentiate between the isomers.

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SUBMITTER: Franski R

PROVIDER: S-EPMC5972171 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Identification of a biliverdin geometric isomer by means of HPLC/ESI-MS and NMR spectroscopy. Differentiation of the isomers by using fragmentation "in-source".

Frański Rafał R Gierczyk Błażej B Popenda Łukasz Ł Kasperkowiak Małgorzata M Pędzinski Tomasz T

Monatshefte fur chemie 20180213 6

<h4>Abstract</h4>A commercially available biliverdin sample was analyzed by means of HPLC/ESI-MS and NMR spectroscopy. It was been found that beside the main IXα 5Z,10Z,15Z isomer, the sample contains also the geometric isomer IXα 5Z,10Z,15E. It was also found the isomers behave differentially upon "in-source" fragmentation in negative ion mode (in contrast to the their behavior upon "in-source" fragmentation in positive ion mode and to their behavior up ...[more]

PMID: 29887645

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Dataset Information

Identification of a biliverdin geometric isomer by means of HPLC/ESI-MS and NMR spectroscopy. Differentiation of the isomers by using fragmentation "in-source".

Abstract

Graphical abstract

Publications

Identification of a biliverdin geometric isomer by means of HPLC/ESI-MS and NMR spectroscopy. Differentiation of the isomers by using fragmentation "in-source".

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