Dataset Information

Effects of medical ozone upon healthy equine joints: Clinical and laboratorial aspects.

ABSTRACT:

Objective

The aim of this study was to verify whether transient inflammatory reactions induced by intra-articular medicinal ozone administration affect joint components, by in vivo evaluation of inflammatory (prostaglandin E2, Substance P, Interleukin-6, Interleukine-1, Tumor Necrosis Factor), anti-inflammatory (Interleukin-10) and oxidative (superoxide dismutase activity and oxidative burst) biomarkers and extracellular matrix degradation products (chondroitin sulphate and hyaluronic acid) in synovial fluid.

Methods

The effects of medicinal ozone were analyzed at two ozone concentrations (groups A and B, 20 and 40 ?g/ml, respectively), using oxygen-injected joints as controls (group C); each group received ten treatments (15 ml gas per treatment). Physical evaluation, evaluation of lameness, ultrasonography, and synovial fluid analysis were performed.

Results

All joints presented mild and transient effusion throughout the study. Group B exhibited the highest lameness score on day 14 (P<0.05), detected by the lameness measurement system, probably because of the higher ozone concentration. All groups exhibited increased ultrasonography scores on day 14 (P < 0.05). Groups A and B exhibited increased proteins concentrations on day 21 (P<0.05). There was no change in hyaluronic acid concentration or the percentage of high-molecular weight hyaluronic acid throughout the experiment. Chondroitin sulfate concentrations decreased in group B, and did not change in group A and C, indicating that neither treatment provoked extracellular matrix catabolism. Cytokine and eicosanoid concentrations were not significantly changed.

Conclusions

The ozonetherapy did not cause significant inflammation process or cartilage degradation, therefore, ozonetherapy is safe at both evaluated doses.

SUBMITTER: Vendruscolo CDP

PROVIDER: S-EPMC5973567 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Publications

Effects of medical ozone upon healthy equine joints: Clinical and laboratorial aspects.

Vendruscolo Cynthia do Prado CDP Moreira Juliana Junqueira JJ Seidel Sarah Raphaela Torquato SRT Fülber Joice J Neuenschwander Henrique Macedo HM Bonagura Giancarlo G Agreste Fernanda Rodrigues FR Baccarin Raquel Yvonne Arantes RYA

PloS one 20180529 5

<h4>Objective</h4>The aim of this study was to verify whether transient inflammatory reactions induced by intra-articular medicinal ozone administration affect joint components, by in vivo evaluation of inflammatory (prostaglandin E2, Substance P, Interleukin-6, Interleukine-1, Tumor Necrosis Factor), anti-inflammatory (Interleukin-10) and oxidative (superoxide dismutase activity and oxidative burst) biomarkers and extracellular matrix degradation products (chondroitin sulphate and hyaluronic ac ...[more]

PMID: 29813093

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Dataset Information

Effects of medical ozone upon healthy equine joints: Clinical and laboratorial aspects.

Objective

Methods

Results

Conclusions

Publications

Effects of medical ozone upon healthy equine joints: Clinical and laboratorial aspects.

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