The RPAP3-Cterminal domain identifies R2TP-like quaternary chaperones.

ABSTRACT: R2TP is an HSP90 co-chaperone that assembles important macro-molecular machineries. It is composed of an RPAP3-PIH1D1 heterodimer, which binds the two essential AAA+ATPases RUVBL1/RUVBL2. Here, we resolve the structure of the conserved C-terminal domain of RPAP3, and we show that it directly binds RUVBL1/RUVBL2 hexamers. The human genome encodes two other proteins bearing RPAP3-C-terminal-like domains and three containing PIH-like domains. Systematic interaction analyses show that one RPAP3-like protein, SPAG1, binds PIH1D2 and RUVBL1/2 to form an R2TP-like complex termed R2SP. This co-chaperone is enriched in testis and among 68 of the potential clients identified, some are expressed in testis and others are ubiquitous. One substrate is liprin-?2, which organizes large signaling complexes. Remarkably, R2SP is required for liprin-?2 expression and for the assembly of liprin-?2 complexes, indicating that R2SP functions in quaternary protein folding. Effects are stronger at 32?°C, suggesting that R2SP could help compensating the lower temperate of testis.

SUBMITTER: Maurizy C 

PROVIDER: S-EPMC5974087 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature