Ontology highlight
ABSTRACT:
SUBMITTER: Waminal NE
PROVIDER: S-EPMC5974128 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Waminal Nomar Espinosa NE Pellerin Remnyl Joyce RJ Kim Nam-Soo NS Jayakodi Murukarthick M Park Jee Young JY Yang Tae-Jin TJ Kim Hyun Hee HH
Scientific reports 20180529 1
Fluorescence in situ hybridization (FISH) is used to visualize the distribution of DNA elements within a genome. Conventional methods for FISH take 1-2 days. Here, we developed a simplified, rapid FISH technique using pre-labeled oligonucleotide probes (PLOPs) and tested the procedure using 18 PLOPs from 45S and 5S rDNA, Arabidopsis-type telomere, and newly-identified Panax ginseng-specific tandem repeats. The 16 developed rDNA PLOPs can be universally applied to plants and animals. The telomere ...[more]