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Imaging individual mRNA molecules using multiple singly labeled probes.

ABSTRACT: We describe a method for imaging individual mRNA molecules in fixed cells by probing each mRNA species with 48 or more short, singly labeled oligonucleotide probes. This makes each mRNA molecule visible as a computationally identifiable fluorescent spot by fluorescence microscopy. We demonstrate simultaneous detection of three mRNA species in single cells and mRNA detection in yeast, nematodes, fruit fly wing discs, and mammalian cell lines and neurons.

SUBMITTER: Raj A

PROVIDER: S-EPMC3126653 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature

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Imaging individual mRNA molecules using multiple singly labeled probes.

Raj Arjun A van den Bogaard Patrick P Rifkin Scott A SA van Oudenaarden Alexander A Tyagi Sanjay S

Nature methods 20080921 10

We describe a method for imaging individual mRNA molecules in fixed cells by probing each mRNA species with 48 or more short, singly labeled oligonucleotide probes. This makes each mRNA molecule visible as a computationally identifiable fluorescent spot by fluorescence microscopy. We demonstrate simultaneous detection of three mRNA species in single cells and mRNA detection in yeast, nematodes, fruit fly wing discs, and mammalian cell lines and neurons. ...[more]

PMID: 18806792

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