Ontology highlight
ABSTRACT:
SUBMITTER: Muller L
PROVIDER: S-EPMC5974325 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Muller Leslie L Fornecker Luc L Chion Marie M Van Dorsselaer Alain A Cianférani Sarah S Rabilloud Thierry T Carapito Christine C
Scientific reports 20180529 1
Sample preparation for quantitative proteomics is a crucial step to ensure the repeatability and the accuracy of the results. However, there is no universal method compatible with the wide variety of protein extraction buffers currently used. We have recently demonstrated the compatibility of tube-gel with SDS-based buffers and its efficiency for label-free quantitative proteomics by comparing it to stacking gel and liquid digestion. Here, we investigated the compatibility of tube-gel with alter ...[more]