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Localization Microscopy of Actin Cytoskeleton in Human Platelets.

ABSTRACT: Here, we measure the actin cytoskeleton arrangement of different morphological states of human platelets using a new protocol for photo-switching of rhodamine class fluorophores. A new medium composition was established for imaging the cytoskeleton using Alexa Fluor 488 conjugated to phalloidin. Morphological states of platelets bound to a glass substrate are visualized and quantified by two-dimensional localization microscopy at nanoscopic resolution. Marker-less drift correction yields localization of individual Alexa 488 conjugated to phalloidin with a positional accuracy of 12 nm.

SUBMITTER: Mayr S

PROVIDER: S-EPMC5979344 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

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Localization Microscopy of Actin Cytoskeleton in Human Platelets.

Mayr Sandra S Hauser Fabian F Peterbauer Anja A Tauscher Andreas A Naderer Christoph C Axmann Markus M Plochberger Birgit B Jacak Jaroslaw J

International journal of molecular sciences 20180411 4

Here, we measure the actin cytoskeleton arrangement of different morphological states of human platelets using a new protocol for photo-switching of rhodamine class fluorophores. A new medium composition was established for imaging the cytoskeleton using Alexa Fluor 488 conjugated to phalloidin. Morphological states of platelets bound to a glass substrate are visualized and quantified by two-dimensional localization microscopy at nanoscopic resolution. Marker-less drift correction yields localiz ...[more]

PMID: 29641438

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