Cytoskeleton dynamics in drug-treated platelets.

ABSTRACT: Platelet activation is a key process in blood clot formation. During activation, platelets go through both chemical and physical changes, including secretion of chemical messengers and cellular shape change. Platelet shape change is mediated by the two major cytoskeletal elements in platelets, the actin matrix and microtubule ring. Most studies to date have evaluated these structures qualitatively, whereas this paper aims to provide a quantitative method of examining changes in these structures by fluorescently labeling the element of interest and performing single cell image analysis. The method described herein tracks the diameter of the microtubule ring and the circumference of the actin matrix as they change over time. Platelets were incubated with a series of drugs that interact with

SUBMITTER: Finkenstaedt-Quinn SA 

PROVIDER: S-EPMC4370791 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature