Ontology highlight
ABSTRACT:
SUBMITTER: Zhang W
PROVIDER: S-EPMC5980232 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Zhang Wen W Walton Travis T Li Li L Szostak Jack W JW
eLife 20180531
The importance of genome replication has inspired detailed crystallographic studies of enzymatic DNA/RNA polymerization. In contrast, the mechanism of nonenzymatic polymerization is less well understood, despite its critical role in the origin of life. Here we report the direct observation of nonenzymatic RNA primer extension through time-resolved crystallography. We soaked crystals of an RNA primer-template-dGMP complex with guanosine-5'-phosphoro-2-aminoimidazolide for increasing times. At ear ...[more]