Complete Genome Sequence of a Novel Recombinant Citrus Tristeza Virus, a Resistance-Breaking Isolate from Uruguay.
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ABSTRACT:
SUBMITTER: Benitez-Galeano MJ
PROVIDER: S-EPMC5981039 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Publications
Complete Genome Sequence of a Novel Recombinant <i>Citrus Tristeza Virus</i>, a Resistance-Breaking Isolate from Uruguay.
Benítez-Galeano María José MJ Vallet Thomas T Carrau Lucía L Hernández-Rodríguez Lester L Bertalmío Ana A Rivas Fernando F Rubio Leticia L Maeso Diego D Vignuzzi Marco M Moratorio Gonzalo G Colina Rodney R
Genome announcements 20180531 22
We report here the complete genome sequence of a <i>Citrus tristeza virus</i> (CTV) from Uruguay, sequenced by using Illumina and Sanger sequencing technology. This CTV DSST-17 genome clustered within genotype resistance breaking (RB) and presents two recombination events. ...[more]
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Project description:The present study for the first time describes biological and molecular characterization of Citrus tristeza virus (CTV) occurring in the Terai area of Uttarakhand State in Northern Himalaya region of India. Direct antigen coated-ELISA and reverse transcriptase-polymerase chain reaction (RT-PCR) detected the CTV infection in Acid lime cv. Pant lemon (Citrus aurantifolia) orchards of Pantnagar with an estimated disease incidence of 16.6-20.5 %. To know the biological and genetic properties, an isolate, CTV Pant 4 was characterized. Isolate Pant 4 could be graft transmitted to Kinnow, Nagpur and Darjeeling mandarins, Mosambi sweet orange, Kagzi lime, Sweet lime, Sour orange but not to Rough lemon. The sequence analyses of the 5'ORF1a (3038 nucleotides) of LPro domain and 3'end (2058 nt) covering ORF7-ORF10 regions of the CTV genome revealed that Pant 4 was closely related to the previously reported Indian CTV isolate, Kpg3 from Northeastern Himalaya region with 97 and 98 % sequence identity, respectively. Whereas, it differed from the previously reported CTV isolate B165 from Southern India with 79 and 92 % identity, respectively for 5'ORF1a and 3' end regions. Recombination and SplitsTree decomposition analyses indicated that CTV isolate Pant 4 was a recombinant isolate originating from Kpg3 as a major and B165 as a minor donor.
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Project description:Citrus tristeza virus strain:RB Genome sequencing and assembly
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