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YAP promotes osteogenesis and suppresses adipogenic differentiation by regulating ?-catenin signaling.

ABSTRACT: YAP (yes-associated protein) is a transcriptional factor that is negatively regulated by Hippo pathway, a conserved pathway for the development and size control of multiple organs. The exact function of YAP in bone homeostasis remains controversial. Here we provide evidence for YAP's function in promoting osteogenesis, suppressing adipogenesis, and thus maintaining bone homeostasis. YAP is selectively expressed in osteoblast (OB)-lineage cells. Conditionally knocking out Yap in the OB lineage in mice reduces cell proliferation and OB differentiation and increases adipocyte formation, resulting in a trabecular bone loss. Mechanistically, YAP interacts with ?-catenin and is necessary for maintenance of nuclear ?-catenin level and Wnt/?-catenin signaling. Expression of ?-catenin in YAP-deficient BMSCs (bone marrow stromal cells) diminishes the osteogenesis deficit. These results thus identify YAP-?-catenin as an important pathway for osteogenesis during adult bone remodeling and uncover a mechanism underlying YAP regulation of bone homeostasis.

SUBMITTER: Pan JX

PROVIDER: S-EPMC5984632 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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YAP promotes osteogenesis and suppresses adipogenic differentiation by regulating β-catenin signaling.

Pan Jin-Xiu JX Xiong Lei L Zhao Kai K Zeng Peng P Wang Bo B Tang Fu-Lei FL Sun Dong D Guo Hao-Han HH Yang Xiao X Cui Shun S Xia Wen-Fang WF Mei Lin L Xiong Wen-Cheng WC

Bone research 20180601

YAP (yes-associated protein) is a transcriptional factor that is negatively regulated by Hippo pathway, a conserved pathway for the development and size control of multiple organs. The exact function of YAP in bone homeostasis remains controversial. Here we provide evidence for YAP's function in promoting osteogenesis, suppressing adipogenesis, and thus maintaining bone homeostasis. YAP is selectively expressed in osteoblast (OB)-lineage cells. Conditionally knocking out <i>Yap</i> in the OB lin ...[more]

PMID: 29872550

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Project description:BackgroundSmall leucine-rich repeat proteins (SLRPs) are highly effective and selective modulators of cell proliferation and differentiation. Podocan is a newly discovered member of the SLRP family. Its potential roles in the differentiation of bovine muscle-derived satellite cells (MDSCs) and its underlying functional mechanism remain unclear. Our study aimed to characterize the function of the podocan gene in the differentiation of bovine MDSCs and to clarify the molecular mechanism by which podocan functions in order to contribute to a better understanding of the molecular mechanism by which extracellular matrix promotes bovine MDSC differentiation and provide a theoretical basis for the improvement of beef quality.MethodsBovine MDSCs were transfected with vectors to overexpress or inhibit podocan, and podocan protein was added to differentiation culture medium. qRT-PCR, western blotting, and immunofluorescence were performed to investigate the effects of podocan on MDSC differentiation. Confocal microscopy and western blotting were used to assess the nuclear translocation and expression of ?-catenin. An inhibitor and activator of ?-catenin were used to assess the effects of the Wnt/?-catenin signaling pathway on MDSC differentiation. We inhibited ?-catenin while overexpressing podocan in MDSCs. Then, we performed mass spectrometry to identify which proteins interact with podocan to regulate the Wnt/?-catenin signaling pathway. Finally, we confirmed the relationship between podocan and Wnt4 by co-immunoprecipitation and western blotting.ResultsPodocan protein expression increased significantly during bovine MDSC differentiation. Differentiation of bovine MDSC was promoted and suppressed by podocan overexpression or inhibition, respectively. Podocan was also shown to modulate the Wnt/?-catenin signaling pathway. Treatment of bovine MDSCs with ?-catenin inhibitor and activator showed that the Wnt/?-catenin pathway is involved in bovine MDSC differentiation. Furthermore, the effect of podocan on bovine MDSC differentiation was suppressed when this pathway was inhibited. We also found that podocan interacts with Wnt4. When Wnt4 was inhibited, podocan-induced promotion of bovine MDSC differentiation was attenuated through Wnt/?-catenin signaling.ConclusionPodocan regulates Wnt/?-catenin through Wnt4 to promote bovine MDSC differentiation.

Dataset Information

YAP promotes osteogenesis and suppresses adipogenic differentiation by regulating ?-catenin signaling.

Publications

YAP promotes osteogenesis and suppresses adipogenic differentiation by regulating β-catenin signaling.

Similar Datasets

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