Ontology highlight
ABSTRACT:
SUBMITTER: Kirkness MWH
PROVIDER: S-EPMC5985029 | biostudies-literature | 2018 Feb
REPOSITORIES: biostudies-literature
Kirkness Michael W H MWH Forde Nancy R NR
Biophysical journal 20180201 3
Force plays a key role in regulating dynamics of biomolecular structure and interactions, yet techniques are lacking to manipulate and continuously read out this response with high throughput. We present an enzymatic assay for force-dependent accessibility of structure that makes use of a wireless mini-radio centrifuge force microscope to provide a real-time readout of kinetics. The microscope is designed for ease of use, fits in a standard centrifuge bucket, and offers high-throughput, video-ra ...[more]